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Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR) for TGF-1 mRNA Total RNA was extracted from frozen lung tissues with TRIzol reagent (TaKaRa Biotechnology Co., Ltd., Dalian, China) and was reverse-transcribed using a reverse transcription kit (TaKaRa Biotechnology Co., Ltd., Dalian, China)

Morris VK, Parikh N, Overman MJ, Jiang ZQ, Maru DM, Elvin D, Kopetz S , Gallick GE

Mol Pharmacol 20, 70914

Although the included studies exhibited a relatively high methodological quality, the number of studies investigating any outcome was limited
