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L., Ehrensaft, D., Vetters, R., Forcier, M

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The dissociation constants for titrations of Mdm2 against FAM-labeled 12/1 peptide were determined by fitting the experimental data to a 1:1 binding model equation shown below: [P] is the protein concentration (Mdm2), [L] is the labeled peptide concentration, r is the anisotropy measured, r 0 is the anisotropy of the free peptide, r b is the anisotropy of the Mdm2FAM-labeled peptide complex, K d is the dissociation constant, [L] t is the total FAM labeled peptide concentration, and [P] t is the total Mdm2 concentration

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