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RNA isolation and RTqPCR RNAs were extracted from USCs or differentiated urothelia using RNAeasy Plus mini kit (Qiagen) and reverse-transcribed with iScript Reverse Transcription Supermix (BioRad)
Histology analysis Processed through fixation in 4% paraformaldehyde, dehydration, paraffin embedding, and precision sectioning at 5 m, lung tissue samples were prepared for histological examination
Because the refractive power of the lens is variable, it permits the diopteric apparatus to focus on objects that are near or far
