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Given its high labor intensity and the availability of alternate assays with equal or better performance (as demonstrated in this study), clonal bisulfite sequencing is not recommended for large-scale validation and biomarker development

doi: 10.3389/fmicb.2017.02146 68 RichardsonNWoottonGEBozwardAGOoYH

Furthermore, it plays an important role in tumor progression by regulating methyl donor metabolism and participating in multiple signaling pathways

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