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This is due to various factors, including poor diets, malabsorption of vitamins, and certain medical conditions and medications which can contribute to lower levels of vitamin B12 in the body
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first application at 30 min after surgery, final 24 h before sacrifice

Injected solution of Fraction A from 3 rd chromatography step and purified on 4 th chromatograpy step, detection: UV = 280 nm
