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10.1046/j.1365-2958.2003.03305.x Mol

For lysates preparation ( 31 ), cultures were centrifuged (10,000 g, 15 minutes), washed twice with potassium phosphate buffer (100 mM, pH 7.0), resuspended in borate buffer (50 mM, pH=8.4), and broken by mechanical lysis with glass beads in a tissue lyser (Qiagen, Courtaboeuf, France) 10 minutes, 50 Hz

Although mitochondria contain about 515% of all the GSH in the cell [34], they cannot synthesize GSH by themselves because they lack GCL [33]

There have been reports of adverse reactions to compounded IV glutathione, often due to impurities or high levels of endotoxins (substances that can cause fever or more serious issues) in poorly manufactured batches