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Histological analyses were conducted under a light microscope (Leica CTR500, Leica Microsystems Inc., Milan, Italy) and images were captured using a high-resolution digital camera (Leica DC300F) at a magnification of 20X

Ras Activation Assay Ras activity in tissues and isolated primary cells was assessed using the Active Ras Detection Kit (Cell Signaling Technology, Danvers, MA, USA) according to the manufacturers instructions

Bauman JN, Frederick KS, Sawant A, Walsky RL, Cox LM, Obach RS, et al
