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In addition, the results of the permutation test illustrated that the OPLS-DA model did not display overfitting phenomenon (Figures 5E,F), and the R2 and Q2 values in the positive and negative ion modes were 0.9097 and 0.165, and 0.9692 and 0.1775, respectively, and the regression line displayed an increasing trend, which indicated that the model had a high degree of accuracy and stability
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