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Final purified proteins were stored in 20 mM Tris-HCl pH 8.0, 150 mM NaCl buffer, unless specified otherwise, and stored at 80 C until further use

The study was carried out in 112 subjects with moderately severe CTS, comparing the effectiveness of a multivitamin B preparation (Vit B6 150 mg, Vit B1 100 mg, Vit B12 500 g daily) for 90 days with a fixed association of ALA (600 mg/day) and gamma-linolenic acid (GLA, 360 mg/day)

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