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Materials and methods HK-2 cell culture HK-2 cells were purchased from The Cell Bank of Type Culture Collection of The Chinese Academy of Sciences, and cultured in DMEM (containing 10% fetal bovine serum, 100 U/ml penicillin and 100 U/ml streptomycin

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In contrast, GHK stimulated the growth of healthy human NIH-3T3 fibroblasts [48]