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To further support our hypothesis that the inhibition of NF-B-DHA binding by 4-OXO-DHA is due to its structural characteristic as a Michael acceptor, which is not shared by other mediators, we incubated 4-HDHA with the above mentioned peptide containing the sequence of the active site of the NF-B p50 protein under identical reaction conditions to those of 4-OXO-DHA