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In summary, GSH enables antibiotic inactivation by serving as a substrate for detoxifying enzymes, such as FosA, and by modulating the expression or stability of antibiotic-degrading enzymes, including the downregulation of NDM-1

PhAN9 (A12M): PhAN9 _A12M_fw, PhAN9 _A12M_rv, PhAN9 (C13S): PhAN9 _C13S_fw, PhAN9 _C13S_rv, PhAN9 (N108H): PhAN9 _N108H_fw, PhAN9 _N108H_rv (Supplementary Table 4) using pANT15 as template

Additionally, our study highlights the absence of specialized motifs within the yeast proteome that are prevalent in higher eukaryotes, such as the WxxW motif 18

Previously identified genetic variations lead to distinct patterns of enzyme production