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Mitochondrial energetic assay The XF Cell Mito Stress Test (#103015-100, Seahorse Bioscience, Copenhagen, Denmark) was used in XF 96 Extracellular Flux Analyzer (Seahorse Bioscience) to measure key parameters of mitochondrial function by directly measuring the oxygen consumption rate (OCR) of live cells

Standardization of Senescence Biomarkers: A major challenge in both the development and application of senescence-targeting therapies is the identification of reliable biomarkers that can accurately assess the presence of senescent cells and their effects.256 Current biomarkers for senescence, such as p16^INK4a, -galactosidase activity, and SASP factors, are useful in research settings, but their clinical applicability remains limited

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