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Description
Organ harvesting, flow cytometry, and cell sorting Mice were euthanized and perfused thoroughly with 30 mL of ice-cold PBS through the left ventricle after incising the right atrium to remove circulating blood from solid organs such as adipose tissue
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270 SarzaniRGiuliettiFDi PentimaCFilipponiASpannellaF
RT-qPCR The other portion of the liver was excised and immediately immersed in RNA-later solution (Ambion, Thermo Fisher Scientific, Waltham, MA, USA) to stabilize the RNA
