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Description
Protein pellets were solubilized in Laemmli buffer and subjected to Western blot analysis as detailed below
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Panminerva Med 45(1):713 Halliwell B (2018) Artefacts with ascorbate and other redox-active compounds in cell culture: epigenetic modifications, and cell killing due to hydrogen peroxide generation in cell culture media
Bottom Line Successful peptide reconstitution requires attention to detail at every step
