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The His 6 -tag was cleaved off by PreScission protease and the resulting authentic 306 amino acid M pro (see Figure S1A in supplemental material) and C300S M pro were further purified by SEC using a HiLoad Superdex 200 pg column (GE Healthcare) in 20 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM DTT

Zhang Y, Xie Z

Dai H, Guzman J, Chen B, Costabel U (2005) Production of soluble tumor necrosis factor receptors and tumor necrosis factor-alpha by alveolar macrophages in sarcoidosis and extrinsic allergic alveolitis

Combination use of human menstrual blood stem cell-derived exosomes and hyperbaric oxygen therapy, synergistically promote recovery after spinal cord injury in rats