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Materials Use Milli-Q-purified water or equivalent for the preparation of all buffers 2.1 pGEX gene fusion construction pGEX vector (see Figure 2) A cDNA or PCR product containing the coding sequence for the protein of interest and appropriate adjacent restriction sites that are compatible with the selected pGEX vector Restriction enzymes, agarose, 1X TAE buffer, T4 DNA ligase Competent E

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BPC-157 stimulated earlier maturation of granulation tissue and resulted in more organized collagen compared to becaplermin treatment