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Digestion was stopped by the addition of 10% FCS and cells were passed through a 70 M and then a 40 M cell strainer to remove tissue remains
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Abnormally elevated levels of DRP1 not only cause mitochondrial fission, mitochondrial fragmentation, and decreased efficiency of mitochondrial ATP production but also impede glucose uptake (104) and glucose-stimulated insulin secretion by inhibiting the translocation of the insulin receptor substrate 1-protein kinase B (IRS1-Akt) pathway and glucose transport proteins in mouse pancreatic -cells (105)
(PubMed) Ebadi M, Montano-Loza AJ
