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Lee MJ, Su YN, You HL, Chiou SC, Lin LC, Yang CC, et al
The samples were processed by pretreatment with 6 M urea for 30 min at 0 C followed by 20% trichloroacetic acid in acetone (wt./v%) at 20 C overnight
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Lysates were cleared by ultracentrifugation at 24,000 g for 30 min at 4 C (Beckman, Ti45 rotor)
