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Further mitochondria also have a role and are associated with cellular senescence, chronic inflammation (inflammaging), decline in innate immunity, and the age-dependent decline in stem cell activity [9] (Fig

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RIP3 siRNA Knockdown Transient genetic silencing of RIP3 was performed by reverse transfection of RAW264.7 cells with 2030 Nm Silencer Select siRNAs (Life Technologies, Inc.) using Lipofectamine RNAiMax reagent (Life Technologies, Inc.), and Opti-MEM medium (Life Technologies, Inc.)