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This association persisted when glycine stimulation was applied in the presence of a glutamate binding site antagonist d-APV, but was blocked by the glycine-site competitive inhibitors indicating that glycine site stimulation alone is sufficient to prime the endocytic process [25, 27]

Mitochondrial membrane potential assessment Mitochondrial membrane potential was determined using Tetrechloro-tetraethylbenzimidazol carbocyanine iodide (JC-1), following the protocols provided by the respective manufacturers (Beyotime, China)