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In total, 50 L of the diluted Matrigel gel was added to the upper chamber of the transwell, gently tilted to ensure even spreading, and incubated at 37 C for 4 h in a cell culture incubator

Free Radical Biology & Medicine

225 SwerdlowR

It is also a co-factor for GPX4-mediated lipid detoxification 35 , and GPX4 activity is necessary for the survival of several tissues, including the liver 36 and kidney 37
