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Peptides were labeled with FITC in the N-terminal and a -alanine residue was used as a spacer between the FITC and the peptide sequence
By international agreement, standardized methods have been used to characterize these enzyme variants, which differ on the basis of biochemical properties such as kinetic activity, electrophoretic mobility, the Michaelis constant for its substrate glucose-6-phosphate and cofactor NADP, the ability to utilize different substrate analogues, heat stability, and pH optima

E., Seth P
