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The mitochondrial genome is more sensitive than the nuclear genome

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Results GSDMD-mediated pyroptosis is activated in ischemic hindlimbs To investigate the involvement of GSDMD-mediated pyroptosis in hindlimb ischemic injury in vivo, we established hindlimb ischemia (HLI) model by ligation of the femoral artery in wild-type (WT) mice

Primary antibodies against SLC7A11 (Cell Signaling Technology, 12691, 1:500 dilution), iNOS (BD Biosciences, 610329, 1:500 dilution), 15LO1 (Abnova, H00000246-D01P, 1:1000 dilution), GPX4 (Abcam, 125066, 1:1000 dilution), POSTN (Santa Cruz Biotechnology, 67233, 1:500), and CCL26 (R&D Systems, AF653, 1:500 dilution) were used