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As long as 60 years ago, identification of the alternative IF-independent mechanism for vitamin B 12 absorption resulted in the discovery that if large enough doses were given, in the range of 100-100,000 g (although only 1% of the cyanocobalamin was absorbed), it was sufficient to provide the daily requirement

Thermal equilibration was then conducted for 20 ps, gradually increasing the temperature from 100 K to 298 K using the Langevin thermostat (collision frequency of 1 ps 1 )

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