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For an amino acid such as alanine, we can identify the two pKa values from its titration curve At the lower pKa (pH 2), both groups are protonated and the net charge is +1

Generally acting as a tumor suppressor, ChaC2 decreases GSH levels in tumor cells, instigates mitochondrial apoptosis and autophagy via UPR and hinders tumor cell proliferation and migration in vitro and in vivo (79)

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Studies using ELISA are strongly encouraged to conduct additional physico-chemical analysis of a subset of perhaps 20% of samples