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His father, a lawyer who was disbarred in 1992, was an addict and separated from Johnsons mother, a substitute teacher, when Johnson was three

In vitro stimulation of human PBMCs with chemical-treated bacteria Human PBMCs were stimulated with bacteria in vitro as described before ( 6 cells/well (respectively 500,000 cells/well for positive and negative control) in IMDM (GlutaMax supplement, Fisher Scientific, Schwerte, Germany) supplemented with 10% fetal bovine serum (FBS, Biochrom, Berlin, Germany), 1 X Pen-Strep Solution (Biowest, Nuaill, France), and 50 M -mercaptoethanol (AppliChem, Darmstadt, Germany) in a 96-well U-bottom microplate (Greiner Bio-One, Frickenhausen, Germany) at 37C in a 5% CO 2 incubator