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Ji Y, Fioravanti J, Zhu W, Wang H, Wu T, Hu J, Lacey NE, Gautam S, Le Gall JB, Yang X, Hocker JD, Escobar TM, He S, DellOrso S, Hawk NV, Kapoor V, Telford WG, Di Croce L, Muljo SA, Zhang Y, Sartorelli V, Gattinoni L

Anesthesiology 128, 921931
At t 0 (after the rehydration process), total RNA was extracted from each animal using the MasterPure Kit (Epicentre), and then used to synthesise cDNA using the SuperScript III First-Strand Synthesis System for RT-PCR (Thermo Fisher), following the manufacturers protocol

Treatment with L-carnitine dramatically lowered blood creatinine and BUN levels in CKD rats, slowed renal hypertrophy, and lessened renal tissue damage
