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Dulbecco's modified Eagle's medium for cell culture, fetal bovine serum, and trypsin were purchased from Gibco (Grand Island, USA)

Statistical comparisons between the baseline and corresponding post-test values were analyzed using Bonferronis test for multiple comparisons following ANOVA

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The research demonstrates that intrinsic factor is not just important but absolutely essential for B12 absorption, with deficiency leading to predictable and severe health consequences that can be prevented through understanding of this physiology