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reported that CK-18 fragments were significantly elevated in the blood of MASH patients and served as an independent predictor of MASH, detecting the presence of MASH with over 90% specificity and an 80% negative predictive value [78]

This was the first demonstration of this finding for CVA21 and is consistent with reports of ideal enterovirus cell culture propagation at sub-physiological temperatures (Yoshii et al., 1977

(Created with biorender.com) Thus, it is questionable whether this mechanism can be applied to other cell types, particularly hepatocytes with their complex, multi-layered antioxidant systems

In particular, the main in vitro studies were reported, describing the principal molecular therapeutic targets and the mechanism of action of the different polyphenols studied