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Metabolomic analysis We used Biocrates AbsoluteIDQ p180 kit to analyze cardiac metabolites from female mice [Young ( n = 7), Old Con ( n = 8), and Old mAb ( n = 7)] with UPLC-MS/MS Xevo TQ, Waters, Pittsburgh, PA, USA) in the Einstein Stable Isotope and Metabolomics Core, according to the manufacturers instructions (BIOCRATES Life Sciences AG, Innsbruck, Austria)

If you get a large bubble, remember you can always just push the liquid back in the vial and try again

Hit gene selection The gene hits were selected according to the following criteria