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The supernatant was discarded, and the sediment containing yeast cells was re-suspended in PBS (0.1 M, pH = 7.4) buffer in such quantity as to obtain approximately 15 10 7 cell/mL, which were examined for metabolic activity (using the resazurin reduction assay), survival rate, and catalase T activity

A key step was the subsequent coating with PEI, which not only enhanced enzyme stability under extreme conditions (such as 60 C, pH 4.5, and 20% isopropanol) but also enabled co-immobilization of the NADH cofactor

The Pracasil Plus base enhances bioavailability while reducing irritation and occlusion-induced maceration

PMID 8125298