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Beads containing bound and reduced Trx1 were resuspended in 6 mL 50 mM HEPES buffer (pH 7.5) and 500 L fractions were loaded on sealable micro gel filtration columns (ThermoFisher Scientific, #69705)
High protein expression of MDS1 and EVI1 complex locus (MECOM) is prognostically adverse and independent of chromosome 3 rearrangements in acute myelogenous leukemia
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