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Senescent cells accumulate over time, meaning that senolytic treatment may need to be repeated periodically, raising concerns about chronic drug administration and potential cumulative side effects.122 Variability in Response and Individual Differences: Senescence is influenced by genetic factors, lifestyle, and environmental exposures, meaning that not all individuals may respond equally to senolytic therapy.123 Age-related changes in metabolism and drug clearance could also impact the effectiveness and safety of senolytics in older adults.124 Dosing and Treatment Scheduling: Determining the ideal dose and treatment schedule for senolytic therapies is complex

Folic acid is contraindicated for use in patients with folic acid hypersensitivity

Ethics Statement The protocol was approved and monitored by the International Guiding Principles for Animal Research, as stipulated by the World Health Organization and as adopted by the Laboratory Animal Centre of Shandong University