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Since a combination of M.tb-specific antibodies as biomarkers to evaluate the M.tb infections showed higher sensitivity and specificity than applying individual antibody approaches (99), developing high-throughput methods with multiplexed measurements may not only be more accurate, but also more cost-effective as disease incidence and/or progression biomarkers due to the long experimental duration and high cost of ELISA in detecting individual antibody isotypes/subclasses to discreet antigens, and their body fluid concentration(s)

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