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It should be highlighted, nonetheless, that significant efforts have already been made to standardize the comet assay score (CAS) for evaluating hazards to human health, as the assays measurement of DNA damage in circulating leukocytes may serve as a predictor of mortality risk

Primers were designed to fit in resulting vector, pJB-HTS upstream of the hexa-histadine tag (5/phos/CCATGGGCAGCAGCCATCATCAT), and downstream (AGCTGGAATTCCTAGTTATTGCTCAGCGGTGGC) (Integrated DNA technologies) of the spacer yielding a fragment containing a phosphorylated 5 end, an initiation codon, a hexa-histadine tag, a thrombin cleavable sequence, a spacer, a termination codon, and an EcoRI site

this effect is particularly evident in premenopausal women

LeeJHBudanovAVParkEJBirseRKimTEPerkinsGAet al