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All target proteins were run on 12% polyacrylamide gels, with the exception of LC3, which was run on a 420% gradient gel (XPO4205, ThermoFisher, Waltham, MA, USA) for clearer separation of the LC3I from LC3II bands

The latest findings further revealed that the HECT domain-containing ubiquitin E3 ligase HUWE1 was also an underlying protective modulator that could combat ferroptosis and abnormal iron accumulation by diminishing the amassing of TfR1, reducing the risk of ALF (Wu et al., 2022)

However, as with any supplement, individual responses vary

Biogenetic and morphofunctional heterogeneity of mitochondria: the case of synaptic mitochondria