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Its not just about adding chemicals

Tanelian and Monroe (37) studied a rabbit model in which they produced corneal nerve fiber injury by a small punch biopsy and subsequently used electrophysiological methods to directly determine the behavior of regenerating small nerve fibers to cold stimuli

Most published findings on both compounds are from rodent models

Each dose draws 0.10 mL, so: 3.00 mL 0.10 mL = 30 doses per vial How long 30 doses lasts depends entirely on frequency which is the whole point of the phase schedule: Practically, roughly one vial per month covers the daily loading phase, and the same vial stretches much further once you drop to maintenance frequency