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Clinical symptoms result from binding of IgM to red cells in vascular beds where the temperature may fall below 30C, such as in exposed fingers, toes, and ears

The homogenate was subsequently centrifuged at 4 C and 3,000 rpm for 20 min, after which the supernatant was collected and stored at -80 C for determining oxidative stress factors and inflammatory factors

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Mn2 + is also an excellent contrast agent for T1-weighted MRI