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However, recent evidence suggests that the gut and oral microbiomes play a significant role in influencing these pathogenic processes

The cultures were collected, their growth measured at optical density (OD) of 600 nm (Spectronic Helios Gamma UV-Vis Spectrophotometer, Thermo Fisher Scientific, UK), centrifuged at 3500 g for 1 min, and the supernatant was collected for measuring TMA, TMAO, BB, carnitine, and choline concentration using LC-MS

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The oral cavity harbors a complex and dynamic microbial community, including both commensal and pathogenic species, many of which are implicated in periodontitis and cardiovascular disease