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Target mRNA levels were normalized to -actin levels using the 2-Ct method [56, 57]

Although they are less toxic than PAP, all these drugs can cause cellular or tissue damage

Hameed, Q

Live labeling of synaptotagmin 1 and EGF receptors For the surface antibody stripping experiments of with Syt1 and EGF, neurons were incubated with 1:100 monoclonal mouse antibodies directed against the lumenal domain of Syt1 conjugated to Atto647N (custom made, Synaptic Systems, Gttingen, Germany), or 1:100 epidermal growth factor (EGF), complexed to Alexa Fluor 647 (#E35351
