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2023) but also synthetic low molecular weight chemicals functioning as iron chelators (Bruzzese et al

Following the addition of ATP, the initial rate of NADH consumption was monitored by measuring the absorbance every minute at 340 nM for 3045 min on a Synergy Neo2 Multi-mode Microplate Reader (BioTek)

This is followed by the release of damage-associated molecular patterns (DAMPs), particularly HMGB1, which in turn causes healthy human bronchial epithelial cells to release more proinflammatory cytokines [54]

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