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In particular, miR-122-5p, which is already established as a critical hepatic biomarker, was upregulated in MASLD serum, consistent with studies in adults

Compared to the people who were not referred, those referred appeared to be older, have higher-grade tumours and be more likely to have abnormal results of tumour IHC testing (Table 4)

Cell culture and assays Total B cells and FoB/MZB were magnetically enriched from mouse spleen using a B cell or MZ and FO B Cell Isolation Kit, respectively (Miltenyi Biotec 130-090-862 and 130-100-366) as per manufacturers protocol

Selecting healthy individuals aged 2040 years without major comorbidities helps minimize variability in plasma composition and supports reproducibility