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Description
The PCR protocol consisted of: a pre-heating step at 95C for 1.5 min, 35 cycles of heating at 95C for 30 seconds, cooling at 64C for 30 seconds, elongation at 72C at a rate of 1 kb/min, and a final elongation step of 5 minutes at 72C

If this sounds familiar, you might be deficient in vitamin B12

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