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After reaching pH 5.0 with the addition of HCl, the solution was firstly deprived by the enzyme TMLD through a Vivaspin centrifugation, then the flow-through was loaded onto a 50 mL Superloop of KTA pure system FPLC and purified using HiTrap 5 mL SP column

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It might be an extension or relapse of adolescent acne (persistent acne) or can be a new occurrence (late-onset acne)

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