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Description
Capillary needles for RNA injection were made using a PC-10 micropipette puller (Narishige), and each oocyte was microinjected with 25 ng of RNA using the Nanoject III (Drummond Scientific), followed by incubation at 16C for 3 d in Modified Barths Solution (EcoCyte Bioscience) with 10 IU ml/ml gentamicin (Invitrogen)
Gradualmente, se ha activado la complementacin con carnitina y poco a poco ha despertado el inters de la comunidad cientfica
Mild nausea in the first 23 injections is common and typically resolves as the body adjusts to elevated methyl donor availability
Weathers Jeffrey G
