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Hepatocytes from mice with liver selective elimination of peroxisomes (liver specific Pex5 deficient mice) displayed severely impaired oxidation of tetradecanedioic acid ( Our unbiased metabolite profiling provided a range of mostly hydrophilic analytes and only a subset of small or amphipathic lipids, which is not unexpected since no single extraction method can cover all metabolite and lipid classes ( 5 Conclusion Our findings confirm that genetic deletion of CROT in normolipidemic mice leads to changes in the liver metabolome consistent with known CROT functions

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