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cDNA synthesis was performed from 1 g of total RNA with the Transcriptor First Strand cDNA Synthesis Kit (Roche) using random hexamer primers
elegans growth 40 , but cysteine limitation reduces synthesis of the protective redox metabolite glutathione 59,60
Experimentally, this problem can be tackled in several ways, the most common being: 1) RNAi knock-down or mutation of the genes encoding the enzymes of the pathway
Neuropsychopharmacology 25 (2), 165172
