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Cells were sorted using a FACS LSRFortessa (BD Biosciences) to determine the percentage of annexin V- and/or 7-AAD-positive cells using a 488 nm laser for annexin V-FITC and annexin V-PE and a 675 nm laser for7-AAD

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Similarly, a significant decrease (49.54%) in reproductive tract weight was documented in the Cyclo-challenged group, matched to those of saline-injected rats