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tested coronary microvascular permeability in a Zucker-DM diabetic rat model, and found increased PKC protein expression and decreased PKC expression in mouse coronary artery endothelial cells ( With respect to PKC specifically, an assortment of studies using animal models and in vitro human endothelial cells taken from a variety of vascular beds show that inhibition of PKC protects microvascular barrier and monolayer barrier functions and decreases trans-endothelial migration ( 3.6 PKC, endothelial inflammation, and atherogenesis in the diabetic vasculature PKC promotes action of inflammatory cytokines and upregulation of endothelial cell adhesion molecules, both of which contribute to a pro-inflammatory and atherogenic microenvironment in diabetic vasculature